Abstract
Phospholipids and polyphosphoinositides were separated by one-dimensional thin-layer chromatography (TLC) on silica gel plates and lipid containing areas were identified by staining with iodine. Difficulties in separating phosphatidylserine and phosphatidylinositol were encountered in the TLC system on silica gel G-25 plates. Among several plates tested good separation of each phospholipid was obtained in the TLC system of a 5% (w/v) (NH4) 2 SO4 impregnated silica gel H plate developed with chloroform/methanol/acetic acid/water (50: 25: 8: 1, v/v/v/v) at room temperature. Triphosphoinositide, which is one of polyphosphoinositides, did not migrate on silica gel G plates with several solvent mixture. Polyphosphoinositides were well resolved from the other phospholipids in the following TLC system: a silica gel G-25 plate developed with 1% (w/v) aqueous solution of oxalic acid were dried at room temperature, actiated at 110°C for 15 min and developed with chloroform/methanol/4N NH4OH (9: 7: 2, v/v/v) after loading the lipids.
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