Abstract
The influence of N?-benzyladenine (BA) on in vitro growth, chlorophyll, rubisco and rubisco activase were studied in tobacco. After 11 weeks of treatment of various concentrations of BA, the most pronounced effect on in vitro growth and chlorophyll was found at 2 μM BA. Rubisco content increased with increasing concentrations of BA, but a point at 2 μM BA was reached beyond which increasing concentrations of BA cause inhibition of this enzyme. Rubisco activity showed patterns of change similar to rubisco content. These data suggest that rubisco activity was associated with an amount of rubisco protein. Under the assumption that effects of BA on rubisco may be related to by rubisco activase, in addition to, its content and activity were determined. The rubisco activase content at 2 μM BA was more increased than other treatments. A similar change pattern was also observed in activity of rubisco activase. These results suggest that the effects of the activation of rubisco by BA seem to be related with rubisco activase.
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