マウスにおける不活化日脳ワクチンのインターフェロン産生能と, 移入免疫脾細胞による感染防御能の検討

Abstract

For the purpose of studying the protective factors to Japanese encephalitis virus (JEV) infection, serum interferon (IFN) activity was measured at different hours after inoculation with inactivated JEV vaccine in cyclophosphamide (CY)-treated and non-CY-treated mice.IFN titer reached its peak between 6 and 12 hours and fell to an undetectable level in 24 hours after inoculation of JEV vaccine. CY treatment (0.25mg/g) did not effect the induction and the course of IFN production.Mortality and HI antibody production in CY-treated mice following intracerebral inoculation of JEV and protective effect of normal or immune spleen cells were studied.Mice transferred with the hyper-immune spleen cells showed lower mortality, but was not significant as compared to those transferred with normal spleen cells. All of the 5 surviving mice transferred with immune spleen cells were positive in serum HI antibody. On the other hand, 4 cases of 5 survived mice non transferred or transferred with normal spleen cells were negative. Among the mice transferred with immune spleen cells, though vaccination was not done, serum HI antibodies appeared within 3 days after transfer of spleen cells in some cases, which is earlier than the time of appearance of HI antibodies in the case of JEV vaccination.