Abstract
The article presents the results of comparing the effectiveness of modern ionization methods used in chromatomass spectrometric analysis: photoionization at atmospheric pressure (APPI) and electrospray ionization (ESI) for the determination of aflatoxin B1 and T-2 toxins related to the main natural pollutants of agricultural feed, food raw materials and food products. Aflatoxin B1 is a member of the aflatoxin family, which in their chemical structure are derivatives of substituted coumarins or furocoumarins. The peculiarity of aflatoxins is that in the process of technological or culinary processing, they are practically not destroyed, while being the strongest hepatocancerogens that cause irreversible liver damage. T-2 toxin refers to trichothecene mycotoxins, in the structure of which there is a trichothecene ring containing a multiple bond and an epoxy group. Most trichothecene mycotoxins are slightly toxic compounds, but T-2 toxin is deadly, having a negative effect on immunocompetent organs, hematopoietic organs, and the gastrointestinal tract, increasing the risk of internal hemorrhage. Due to the impossibility of preventing contamination of agricultural feed, food raw materials and food products with mycotoxins, their strict control is necessary. Chromatomass spectrometry is one of the methods that provide high sensitivity and reliability for the determination of mycotoxins Methods of ionization sources, such as electrospray and photoionization at atmospheric pressure during mass spectrometric detection of mycotoxins by high-performance liquid chromatography (HPLC-MS/MS), are evaluated. In order to increase the sensitivity of the determinations, the optimal conditions of the quadrupole-time-of-flight mass spectrometric detector were selected and the maximum intensity responses of the analytes were obtained. During the experiment, it was found that the intensity of the signal with a photoionization source obtained by analyzing the T-2 toxin is higher than the signal obtained by using an electrospray ionization source.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
