Abstract
Several symbiotic mushroom fungi (Tricholoma caligatum, Tricholoma robustum, Lyophyllum shimejii, Rhizopogon rubescens and Lactarius hatsudake) were inoculated to in vitro cultured trees, Japanese black pine and Japanese red oak. The inoculation method was crucial for covering of fungi over the root of in vitro plantlets. Dipping the root in the blended liquid culture of fungi was better than setting the agar block of fungi culture on the root. We found the mantle formation of Rhizopogon rubescens and Lactarius hatsudake around the roots of Japanese black pine and Lyophyllum shimeji around those of Japanese red oak in vitro.
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