ГЕМАТОЛОГІЧНІ ТА МІКРОБІОЛОГІЧНІ ПОКАЗНИКИ, СТАН ПРИРОДНИХ МЕХАНІЗМІВ ЗАХИСТУ У КОРОПА ЗА ДІЇ ПРЕПАРАТУ «ФЛЮМЕК»

Abstract

In the article presented results of own researches on the impact of antibacterial drug «Flyumek» on microbiological, hematological and immunological parameters of fish.The study was conducted in three groups of healthy age–2 carp, it was six animals each groups. The first group – research, through which the probe within 7 days of antibiotic «Flyumek» at 10 mg/kg of fish with 3% starch suspension, the second experimental group – 20 mg/kg of fish, the third group – control, received only 3% in starch suspension. Fish was kept in special trays under conditions of continuous closed system of water circulation. The material for the research served fish blood, which was taken after the experiment.For determine the sensitivity of pathogenic microorganisms of fish to the drug «Flyumek» was taken biological material from external integument, gills and internal organs (kidneys) carp with signs of the most common infectious diseases – Aeromonas infection. It was found a high antibacterial activity of the drug – diameter zone growth retardation culture around the disc was 25 mm.Investigated hematological parameters, particularly the number of red blood cells and hemoglobin concentration in the blood of carp experimental and control groups were within standard values for the age and species of fish. Not recorded probable changes in the number of red blood cells and hemoglobin concentration in the blood of experimental carp groups compared to control. This suggests no violation of the oxygen–transport function of red blood cells and hemolysis .Was studied parameters characterizing humoral nonspecific resistance – lysozyme and bactericidal activity of serum and content of circulating immune complexes. Stated that the fish that received study drug in an amount of 10 mg / kg, the content of circulating immune complexes was 51.7 ± 1.78 mmol/l and virtually no different from the control group, where their content – 50.3 ± 1.78 mmol/l, and the application of higher doses – 20 mg/kg, recorded an increase in their level to 60.3 ± 2.16 mmol/l, indicating a tendency to increase the antigenic load on the carp body . As a result dose of the drug «Flyumek» 10 mg/kg caused increase lysozyme and serum bactericidal activity of fish according to 10.0 (p < 0.05) and 11.5%. However, the use of larger doses – 20 mg/kg caused a downward trend bactericidal activity, but did not significantly affect the activity lysozyme serum.The results of the research indicate a high antibacterial activity of the drug «Flyumek» and no immunosuppressive action of investigational drug on humoral non–specific resistance of the organism carp. In this case, the drug dose of 10 mg/kg causes immune–stimulating effects on the natural defense mechanisms of fish.