ПОЛИМОРФИЗМ ГЕНА NOD2 У ДЕТЕЙ С РЕКУРРЕНТНЫМИ РЕСПИРАТОРНЫМИ ИНФЕКЦИЯМИ

Abstract

The highest incidence rate is recorded among children with recurrent respiratory infections (RRI). It has been shown that changes in the NOD2 gene, which encodes the innate immunity receptor, can be factors in susceptibility to viral pathogens. The aim of the study was to analyze the polymorphism of the NOD2 gene in children with RRI. Materials and methods of research: a single-center cohort observational retrospective cohort study was carried out. The study was conducted among children aged 1–18 years. The study included 500 patients with an annual number of proven RRI episodes of at least 6 in the last year in relation to the date of the start of the study (10.01.2017–12.31.2019; mean age 15±2.7 years, male sex 51.2%) and 100 conditionally healthy children included in the control group (mean age 12±2.6, male sex 48.0%). Leu1007fsinsC, Cly908Arg, and Arg702Trp, polymorphic variants of the NOD2 gene were determined by the allele-specific polymerase chain reaction (PCR). Results: it was found that in children with the insertion of the nucleotide «C» (Leu1007fsinsC), the chance of developing RRI is higher compared to other NOD2 gene polymorphism. Age-specific features of the occurrence of the Leu1007fsinsC polymorphism of the NOD2 gene in patients with the N/insC genotype were revealed. Conclusion: there were no statistically significant differences in the frequency of occurrence of polymorphic variants Arg702Trp (2104 С>T) и Gly908Arg (2722G>C) of the NOD2 gene in children with RRI compared with the control group. The predisposition of children to RRI may be associated with a Leu1007fsinsC polymorphisms of the NOD2 gene. These data require further study and may be useful for the development of new approaches to personalized RRI therapy, since the use of pattern recognition receptors (PRRs) agonists and drugs that modulate PRR-mediated signaling pathways is the most rational therapeutically effective approach for children with RRI.