기수산 물벼룩(Diaphanosoma celebensis)의 대량생산을 위한 대체 먹이원의 효과

Abstract

This study examined the effect of formulated diets (composed of condensed freshwater <TEX>$Chlorella$</TEX> or <TEX>$Schizochytrium$</TEX> sp., <TEX>$Crypthecodinium$</TEX> sp., and EPA oil sources derived from baker's yeast) on the population growth and fatty acid composition of the brackish water flea (<TEX>$Diaphanosoma$</TEX> <TEX>$celebensis$</TEX>) to determine diet effectiveness for mass production. Results indicated that the highest maximum density, fecundity, and population specific growth rate (SGR) of the flea were observed using a diet of <TEX>$Tetraselmis$</TEX> <TEX>$suecica$</TEX> (control). However, results using condensed freshwater <TEX>$Chlorella$</TEX> and the mixed treatment of <TEX>$Schizochytrium$</TEX> sp.+<TEX>$Crypthecodinium$</TEX> sp.+EPA oil did not significantly differ from the control treatment (<TEX>$P$</TEX>>0.05). Thus, <TEX>$T.$</TEX> <TEX>$suecica$</TEX> could be replaced with a combination diet of <TEX>$Schizochytrium$</TEX> sp., <TEX>$Crypthecodinium$</TEX> sp., and EPA oil in condensed freshwater <TEX>$Chlorella$</TEX> without growth interruption of <TEX>$D.$</TEX> <TEX>$celebensis$</TEX>. As the percentage of combination diet (mix of <TEX>$Schizochytrium$</TEX> sp., <TEX>$Crypthecodinium$</TEX> sp., and EPA oil) in condensed freshwater <TEX>$Chlorella$</TEX> increased, the level of ARA, EPA, DHA, and n-3 HUFA in <TEX>$D.$</TEX> <TEX>$celebensis$</TEX> also increased. However, the population density of <TEX>$D.$</TEX> <TEX>$celebensis$</TEX> sharply decreased with the use of treated supplements C5M5 (CHL [50%] and <TEX>$Crypthecodinium$</TEX> sp.+ <TEX>$Schizochytrium$</TEX> sp. + EPA oil [MIX 50%]). The optimum proportions of CHL and MIX for <TEX>$D.$</TEX> <TEX>$celebensis$</TEX> within the mixture were 70% and 30%, respectively. Based on the results, the combination of <TEX>$Schizochytrium$</TEX> sp., <TEX>$Crypthecodinium$</TEX> sp., and EPA oil in condensed freshwater <TEX>$Chlorella$</TEX> appears to be a stable and effective food source for mass production of <TEX>$D.$</TEX> <TEX>$celebensis$</TEX>.