Abstract
The observation of lignin distribution in the cross section of Acacia chips and the digester rejects in kraft pulping was carried out by using EPMA (Electron Probe Microanalyzer) with the newly developed procedure of Osmium-tagging to lignin, presented in the previous report. The distribution pattern of lignin can be detected clearly by the distribution and concentration of the Osmium tagged to lignin. STEM-EDS (Scanning Transmission Electron Microscope-Energy dispersive X-ray Spectrometer) technique was also applied to observe more precisely the distribution of the Os-tagged lignin in wood components. The observed results are summarised as follows ;(1) More vessels, which were thickly surrounded by vasicentric parenchyma cells, were observed in the cross section of the KP rejects than in that of the chips.(2) It was found that the lignin concentration is high in these vasicentric parenchyma cells for the KP rejects and on the other hand it is high in ray parenchyma cells for the normal chips. High Osmium concentration in parenchyma cells may be thought to depends not only lignin but on resinous substances which can not be extracted with alcohol benzene.(3) STEM-EDS analysis revealed that in the KP rejects fiber cell walls are considerably deformed and delignified but firmly combined with lignin rich middle lamella.(4) The distribution of the lignin in the cross section of the KP reject resembled that of the chips near the knots. Accordingly, it is considered that the difficulty of digesting of chips much depend on the distribution of lignin in the chips.
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