Boron reduces aluminum deposition in alkali-soluble pectin and cytoplasm to release aluminum toxicity

Abstract

Boron (B) is indispensable for plant growth and has been reported in the mitigation of aluminum (Al) toxicity in different plants. This study focused on the efficacy of B in reducing Al toxicity to trifoliate orange seedlings in a hydroponic experiment. Boron supply had a positive effect on root length and plant growth-related parameters and attenuated Al-induced inhibition of plasma membrane H+-ATPase activity. X-ray photoelectron spectroscopy (XPS) in conjunction with scanning electron microscope-energy dispersive x-ray spectrometer (SEM-EDS) revealed that B reduced Al accumulation in root cell wall, especially on pectin fractions (alkali-soluble pectin), accompanied by suppressing pectin synthesis, pectin methylesterase (PME) activity and PME expression. Furthermore, B application inhibited NRAT1 expression while increased ALS1 expression, indicating restraining Al transport from external cells to cytoplasm and accelerating accelerating vacuolar sequestration. The results were further demonstrated by transmission electron microscope-energy dispersive x-ray spectrometer (TEM-EDS) analysis. Taken together, our results indicated that B mainly promoted the efflux of H+ by regulating the plasma membrane H+-ATPase activity, and reduced the demethylation of pectin to weaken Al binding to carboxyl. More importantly, B alleviated some of the toxic effects of Al by compartmentalizing Al into vacuoles and decreasing the deposition of Al in cytoplasm.