ＡＣＤ液，ＣＰＤ液による全血および赤血球濃厚液の経時的変化（代謝性変化および機能性変化）

Abstract

This study was conducted to compare the functional and metabolic changes and their relationship in ACD and CPD preserved whole blood (WB) and red blood cells (RBC), i. e., formally called packed red blood cells.200ml of blood was collected into blood collecting plastic bags containing 30ml of ACD or 28ml of CPD solutions from 6 young healthy male donors respectively. Each blood sample was equally divided into two parts; One part was used to make a preserved WB and the other part was further processed into RBC. Thus 4 types of samples were obtained, such as ACD·WB, ACD·RBC, CPD·WB, CPD·RBC. They were stored at 4°C during experiment. Measurements of the following parameters were performed within 3 hours and 3, 7, 10, 14 and 21 days after the collection of the blood. Measured parameters and their methods were; Serum glucose by the o-TB method, 2, 3-DPG, serum pyruvate and serum lactate by the enzymatic UV methods, serum K and Na by the flame photometric methods, serum Cl by Schales and Schales method, erythrocyte osmotic resistance by the coil planet centrifuge method and oxygen dissociation curves by using Hemox-Analyzer®.The results obtained from this experiment were summerized as follows;1) The metabolic activities were found in the following order; CPD·WB>CPD·RBC>>ACD·WB≈ACD·RBC.2) The changes in oxygen dissociation curves were in parallel relationship with the changes in 2, 3-DPG. Their changes were inversely proportional with the metabolic activities.3) The changes in serum electrolytes depend on whether they were WB or RBC rather than the types of preservative solution used.4) Erythrocyte osmotic resistance was maintained slightly better in the CPD preserved two groups.5) Metabolic activity, function of Na-K pump and membrane fragility were not always in parallel relationship with one another.6) Although the concentration of serum K in RBC was extremely high compared with that in WB, the amount of serum K in both types of preserved blood were almost the same. Therefore, difference in the concentration of serum K does not likely cause a significant difference in clinical effects.