Abstract
Morphological changes in corneocyte were investigated in relation to skin aging. Corneocyte was sampled with the detergent scrub technique using Triton X-100 and stained with a mixture of methylene blue and rhodamine B. The following parameters were measured; surface area, perimeter, shape factor, nuclear inclusion, and numerical counts. Surface area and perimeter of corneocyte increased with age (r=0.838 for male, r=0.73 for female between surface area and age; r=0, 823 for male, r=0.725 for female between perimeter and age), however, there was no direct relationship between shape factor and age. In males aged over 50 years, corneocyte surface area was 1.42 times larger than that under 9 years, and in females aged over 50 years surface ares was 1.34 times larger than that under 9 years. These observations suggest that the morphological parameters of corneocyte could be very useful to assess skin aging.
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