АНАЛИЗ РЕГЕНЕРАЦИОННОЙ АКТИВНОСТИ VITIS VINIFERA В УСЛОВИЯХ IN VITRO

Abstract

Obtaining healthy planting material valuable in terms of economic characteristics of agricultural crops is an advanced task for import substitution and development of own production of high-quality products. The technology of cells and tissues in vitro by the method of clonal micropropagation makes it possible to improve the health of plant material and replicate it in large volumes without changing the genotype of the culture. At the same time, an important component is the regenerative activity of plants, the individual factor is different depending on the breed and variety. The research was conducted in the laboratory "Biotechnology of Agricultural Plants" of the Chechen State University named after A.A. Kadyrov in 2021-2022. The main purpose of the research was to study the regenerative activity of micro-runs of grape varieties obtained in vitro conditions by the apical meristem method. The dynamics of the formation and development of the root system of table grape varieties, the reproduction rate and other parameters at each stage of the technology of microclonal reproduction in vitro conditions are studied. The results of plant development in vitro conditions indicate a high percentage of plant rooting. When studying and evaluating the regenerative activity of Vitis Vinifera, it was found that all the studied varieties are sufficiently rooted after 50-55 days in sterile conditions. By this time, it is possible to transfer microplants to adapt to in vivo conditions, with an average survival rate of 95%. The results obtained in the studies can be used to work with cell and tissue culture in the biotechnology of fruit crops and grapes, as well as in fruit growing and nursery to obtain healthy and homogeneous planting material.