Кариопатическое действие биопрепарата Bacillus subtilis 12В на состояние сперматогенного эпителия животных при воздействии гельминтов

Abstract

Objective of research: to study the caryopathic effect of bio-preparation Bacillus subtilis
 12В on the status of spermatogenic epithelium of white mice after a single intra-abdominal
 administration of the Fasciola hepatica extract.
 Materials and methods: The investigations were conducted on white male mice after oral
 use of preparation Sporovite based on B. subtilis 12B; then the intra-abdominal injection of F.
 hepatica extracts at a dose of 100 mkg/head was applied. The protein extract from F. hepatica
 was administered to mice of the second group, and animals of the third group received only
 the probiotic Sporovite. Animals of the fourth group did not get the preparation and served as
 controls. 48 hours later the animals were killed; touch smears obtained from testis were stained
 by the Romanovsky method and examined under a microscope what enables to determine the mitotic index and the number of pathological meiosis forms. The experiments were conducted
 on lambs whose seminal vesicles were placed into a solution of 10% Formalin and examined
 histologically. 2-3μ-thick slices were stained with Haematoxylin and Eosin by Van Gieson method
 and examined under a microscope at 50, 400 and 1000 x magnifications.
 Results and discussion: During the pathomorphological and caryomitotic studies of testis
 of white mice and lambs after administration of Sporovite on the background of intra-abdominal
 injection of F. hepatica extract the reduction of negative effects on the status of spermatogenic
 epithelium of testis in animals wasn’t observed. A decrease in mitotic index by 2-3 times and a
 high amount of pathological forms were registered. The number of metaphases with preterm
 chromosome disjunction under the joint effect of F. hepatica and B. subtilis extracts has decreased
 by half.