Морфофункциональные изменения клеток Bacillus cereus под воздействием наночастиц металлов и оксидов металлов

Abstract

The results are presented of the comparative study of the presence of characteristic features of disorders of the microstructural organization of the test strain B. cereus ATCC 10702 cells by stable nanoparticles of silver, gold, platinum, zinc oxide and titanium dioxide with loss of cell viability during their interaction with nanoparticles in an aqueous medium using methods of bacteriology and electron microscopy. General size-dependent features of the effect of nanoparticles on B. cereus ATCC 10702 cells and differences associated with both the adsorption of metal oxide and noble metal nanoparticles on the surface of bacteria and the loss of viability of bacterial cells with different levels of their destruction, reaching 67-70 % in the case of nanoparticles noble metals, were found. The chemistry of the effects of silver, gold, platinum and zinc and titanium oxides nanoparticles on the test microbe B. cereus ATCC 10702 is considered. The assumption is made that the main feature of the interaction of nanodispersed structures based on noble metals and metal oxides is not associated with an increase in the specific contact surface of the dispersed phase, but with a significant increase in the specific surface energy, which leads to the modification of the multilayer hydrated water shells surrounding nanoparticles. The measured zeta (ζ-)-potential of metal and metal oxides nanoparticles characterizes the total effect formed on the diffusion-mobile boundary of the nanoparticle; its value is a quantitative characteristic of the equilibrium energy state of dispersed systems acting as biocatalysts of intracellular enzymes and causing hydrolysis of the polymer structures of cytoplasmic membranes and cell membranes and ultimately the death of microbial cells. The obtained experimental data can be used in creation effective antimicrobial compositions.