Abstract
Acacia honey, which was collected from nectar of Robinia pseudoacacia by honeybee, is produced more than any other honey in Korea. It is known to have antibacterial activity against Helicobacter pylori that induced gastric disease. In present study, quantitative analysis for abscisic acid which was reported as anti-H. pylori compound in acacia honey was developed and validated by UPLCDAD. The UPLC method was established using Halo C18 column (2.1×100mm, 2μm) that eluted gradient system by acetonitrile and 0.1% phosphoric acid at 263nm of detection wavelength. The method was completely validated by specificity, linearity, limit of detection, limit of quantification, precision and accuracy. Also, the established UPLC method was possible to quantitative analysis for abscisic acid in Korean acacia honeys collected from 8 different areas. These results suggest that the developed UPLC method could be sufficiently applied for quality evaluation of Korean acacia honeys.
Published Version
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