구제역 바이러스 혈청형 A와 O 에피토프 재조합단백질 백신의 구제역 방어 효능

Abstract

Foot-and-mouth disease (FMD) is a highly contagious viral disease susceptible to cloven- hoofed animals such as cattle, pig and goat, consequently exerting a bad influence on the livestock industry. Although various FMD vaccines have been developed for several decades, the efficacy of current FMD vaccines is restrictive because of the FMD virus (FMDV) diversity. The aim of this study was to determine whether the FMD virus (FMDV) serotype A and O epitope recombinant protein vaccine shows protective effects in a FMD animal model using pigs. To manufacture the recombinant protein vaccine, three or six replicates of VP1 epitopes of A/Pocheon/SKR/2010 and O/Andong/SKR/2010 FMDV strains were used and coupled with a swine immunoglobulin G heavy-chain constant region. Pigs were vaccinated twice (interval of 4 weeks) intramuscularly and examined the serum titers of FMDV specific antibody and neutralization antibody. After FMDV challenge, the gross lesions and clinical signs in FMD were assessed, and the viremia was measured by RTPCR assay using oral swabs. In the field experiment using pigs, FMDV-specific antibodies were markedly induced in the sera of FMD vaccine-immunized pigs. In addition, produced antibodies dramatically inhibited FMDV in vitro. Moreover, from challenge test in pigs to test the potency of FMD vaccine, at least more than 93% of vaccinated pigs were clearly protected from FMD. Taken together, these results strongly demonstrate that FMDV bivalent epitope-constituted recombinant protein vaccine shows obviously protective effects against two different serotypes (A, O) of FMDV.