Морфологические особенности дрожжей рода Saccharomyces в процессе адаптации к экстремальным значениям глюкозы и этанола

Abstract

The influence of extreme values of glucose and ethanol on the morphological properties of the yeast of the genus Saccharomyces was studied. The ability of strains S. cerevisiae Y-503, S. cerevisiae DAW-3a, and S. oviformis M-12X to adapt to stress was found. The dependence of the functional state and morphological properties of the studied strains on ploidy was shown. It was established that the cell forms of S. cerevisiae Y-503 and S. oviformis M-12X were diverse, the cells of the haploid strain S. cerevisiae DAW-3a were characterized by a round shape and smaller sizes compared to the polyploid strain Y-503 and diploid M-12X. Adaptation to different concentrations of glucose was accompanied by a change in the shape of the cells Y-503 and DAW-3a, while the sizes of cells were practically un-changed. Under conditions of simultaneous osmotic and ethanol stress in the cells of the studied strains, the presence of granular cytoplasm was revealed, which is associated with the accumulation of reserve nutrients. A change in the shape of the cells, form, surface, profile, color, size of colonies was detected. The study of the properties of the yeast of the genus Saccharomyces in the process of adaptation to extreme conditions may serve as the basis for the selection of strains that are promising for use in food biotechnology.