ОСОБЕННОСТИ ПОЛУЧЕНИЯ И СОХРАНЕНИЯ CANNA × HYBRIDA HORT. EX BACKER В УСЛОВИЯХ IN VITRO

Abstract

The aim of the study was to investigate means of morphogenesis and explant regeneration characteristics in in vitro culture and to develop biotechnological methods for micropropagation and conservation of promising garden varieties of Canna. The experiments were conducted in the plant biotechnology and virology laboratory of the Nikitsky Botanical Garden, National Centre of Science. For the induction of adventitious shoot development of explants, a Murashige and Skoog (MS) nutrient medium was used having 30 g / l of sucrose, 10 g / l of agar-agar with BAP and TDZ growth regulators. For the in vitro deposition, explants (vegetative buds and meristemoids) were placed on a modified MS medium having 60 g / l of sucrose and 0.2-0.4 g / l of CCС. The culture vessels with explants were transferred to cooling chambers with a low positive temperature (2-6 ° C) and an illumination of 0.1-0.5 klx. It was found that for active adventitious shoot formation during cultivation of Dar Vostoka and Suevia explant varieties, it is necessary to add 1.27 mg / l of TDZ to the MS nutrient medium, and 1.91 mg / l of TDZ for the Livadia variety. It is shown that for the deposition of meristemoids of Dar Vostoka, Livadia and Suevia varieties, the nutrient medium of ½ MS with 0.15-0.25 g / l of CCC is optimal; for adventitious shoots of the Suevia variety, the optimal nutrient was ½ MS with 0.2 g / l of CCC.