Abstract
The aim of the study is to test the antibiotic activity of representatives of the Bacillaceae family against the mycelial fungus Rhizopus stolonifer Vuillemin (1902). The strains of Bacillus altitudinis, Bacillus atrophaeus, Bacillus cereus, Bacillus megaterium, Bacillus simplex, Bacillus subtilis, Peribacillus simplex and Bacillus sp. were used, isolated by the authors from agricultural soils of the Krasnoyarsk Region and in previous studies showing antagonism against phytopathogenic fungi Fusarium spp., Alternaria spp., Sclerotinia sclerotiorum, Geotrichum candidum, as well as against seed mold pathogens of the genus Aspergillus, Penicillium and Mucor. The antibiotic activity of the studied strains against R. stolonifer was assessed in Petri dishes using the counter culture method. The area of the R. stolonifer colony in the presence of the antagonist strain as a percentage of the control variant (without the antagonist) after 10 days of incubation at a temperature of (25 ± 1) °C was used as an indicator of antibiotic activity. Of the 21 tested strains, 4 did not exhibit antagonism towards R. stolonifer; 16 strains showed weak or moderate antibiotic activity (reduction in the area of fungal colonies by 29.1–79.1 % relative to the control); 1 strain showed high antibiotic activity (reduction in the area of R. stolonifer colonies by 92 % relative to the control). No relationship was found between the antibiotic activity of the studied strains towards R. stolonifer and the taxonomically close zygomycete Mucor sp. (Spearman's rank correlation coefficient Rs = 0.272, statistically insignificant). Based on the results of the studies, the B. atrophaeus RSA9 strain can be proposed as an agent for the biological method of controlling R. stolonifer.
Published Version
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