Abstract
The influence of hyperthermia (33 ºC, 2 days) on the ultrastructure of palisade cells of mesophyll of the first rosette leaves of arabidopsis Columbia 0 ecotype (Col-0, phases 1.02–1.04) was studied. Samples of 12-day-old seedlings were selected in 2 variants: control and 2 days 33 ºC. Seedlings of the control variant were grown in a growth chamber with a photoperiod of 15/9 hours. (day/night), illumination 5.5 klx, 75 % humidity and temperature 22 ºC. In the experimental variant containers with 9-day-old seedlings were transferred for 2 days to a growth chamber with a preset light 5.5 klx and temperature 33 ºC, with a photoperiod of 15/9 hours. The conducted ultrastructural analysis allowed to reveal the spectrum of rearrangements of palisade cells after two-day action of high (33 ºC) temperature. It was shown that the high temperature negatively affected size of mesophyll palisade cells, the cross-sectional area of which was 12 % smaller than in the control. Chloroplasts show an increase in granality: in the control granas contained 6–10 thylakoids, often combining into larger granas, up to 20 or more thylakoids in the intersection zone, while after two-day hyperthermia the granas contained 20 or more thylakoids, often forming giant granas of 60 and more thylakoids, the average cross-sectional area of starch granules decreased by almost half: 0.99 μm2 compared to 1.92 μm2 in the control, the diameter of plastoglobuli increased 3–4 times: to 100–200 nm compared to 30–50 nm in the control. In mitochondria, there was a decrease in the partial volume of the cristae, enlightenment of the matrix, the cross-section of mitochondria increased at least twice: 1 μm2 compared to 0.44 μm2 in the control. The mean cross-sectional area of peroxisomes also increased at least twice, to 1.36 μm2 compared with 0.77 μm2 in the control.
Highlights
На уровне ландшафтного проекта рассмотрены возможности создания учебных аудиторий на открытых пространствах Киевского национального университета имени Тараса Шевченко
The article deals with estimation on project level the possibilities of creating several outdoor classrooms for the Taras Shevchenko National University of Kyiv
Taras Shevchenko National University of Kyiv has the opportunity to create a number of classrooms in open spaces
Summary
З'ясовано вплив гіпертермії (33 °С, дві доби) на ультраструктуру палісадних клітин мезофілу перших справжніх листків арабідопсису екотипу Columbia 0 (Col-0, фази 1.02–1.04). Що висока температура негативно впливала на розміри палісадних клітин мезофілу, площа перерізу яких була на 12 % меншою, ніж у контролі. У хлоропластах показано зростання гранальності: у контролі грани містили 6–10 тилакоїдів, часто об'єднуючись у більші грани, до 20 і більше тилакоїдів у зоні перетину, тоді як за дводобової гіпертермії грани містили 20 і більше тилакоїдів, часто утворюючи гігантські грани із 60 і більше тилакоїдами, майже вдвічі зменшувалася середня площа перерізу гранул крохмалю: 0,99 мкм2 порівняно із 1,92 мкм2 у контролі, діаметр пластоглобул зростав у 3–4 рази до 100–200 нм порівняно із 30–50 нм в контролі.
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More From: Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology
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