ЕКСПЕРИМЕНТАЛЬНЕ ВИЗНАЧЕННЯ ЕФЕКТИВНОЇ ДОЗИ (ЕД50) СУХИХ ЕКСТРАКТІВ ЛИСТЯ ТА КОРЕНЕВИЩ IRIS HUNGARICA З ПОТЕНЦІЙНИМИ АНАБОЛІЧНИМИ ВЛАСТИВОСТЯМИ

Abstract

Keywords: effective dose, anabolic activity, extract, hungarian iris. Acknowledging the importance of plants in the correction of negative nitrogen balance and mild to moderate muscular atrophy, the following advantages of herbal medicinal products over synthetic drugs were taken into account: optimal bioavailability at the systemic, organ and cellular levels; biochemical digestibility of intermediate and end products of metabolism, which decreases the probability of accumulation significantly; organotropic, allergenic and toxic action. The study objective was to determine ED50 values of Iris hungarica leaf and rhizome dry extracts by application of the least square method to the anabolic activity in the intact animals. The study was conducted in 72 white outbred male rats, weighing 230 to 240 g. The anabolic activity of the extracts is estimated by the following indicators: body weight gain of animals; relative weight of internal organs – heart, gastrocnemius muscle, testes; total protein in blood serum, gastrocnemius and cardiac muscles. The experimental animals were administered extracts in doses of 50 mg/kg, 100 mg/kg, 150 mg/kg, 200 mg/kg, 250 mg/kg, and 300 mg/kg. The mean effective dose was 148.81±23.91 mg/kg for Iris hungarica dry leafextract, and 147.27±26.11 mg/kg for Iris hungarica dry rhizome extract as determined by signs of the anabolic effect in the intact animals. Thus, the presence of anabolic effect in dry extracts of leaves and rhizomes of Hungarian iris shows the potential of herbal medicinal products, and the need for more pharmacological studies in order to create a new medicinal product for the correction of negative nitrogen balance and mild to moderate muscular atrophy.