Спосіб терапії кролів за гонадодистрофії

Abstract

The article presents the results of gonadodystrophy therapy method in rabbits. Studies were carried out on sexually mature rabbits, which belonged to private farms in the Kharkov region. Our method of diagnosis and therapy of gonadodystrophy included clinical-andrological, biochemical (protein, vitamin A, dynamics of prooxidant-antioxidant status), thermographic and ultrasonographic studies, with the appearance of postocytopathograms and the use of computer programs. Method of treatment involves oral administration of the drug «Karafand+OV», which contains carotenoids, phytoandrogens and gadolinium orthovanadate nanoparticles activated by europium. We have determined the pharmacotherapeutic effectiveness of the drug «Karafand+OV». After administration to rabbits, the concentration of vitamin A increased significantly by 1.68 times; positive changes were detected in the dynamics of the prooxidant-antioxidant status: the concentration of malate dialdehyde in serum and erythrocytes decreased, the concentration of catalase in serum and erythrocytes increased significantly. In addition, the positive effect of the drug on the structure and function of the testicles was noted: the diameters of the convoluted tubules and the area of the interstitial tissue increased; increased the number of Leydig cells, increased the area of cells and their nuclei; the nuclear-cytoplasmic ratio has increased. Characteristic were changes in the post-cytogram of rabbits, so after the drug administration, the number of epithelial cells, leukocytes and microorganisms decreased. The ratio of epithelial cells and leukocytes was approximately the same, but the ratio of epithelial cells to normal structure in dystrophic ones changed. The histological cuts of the testes of the rabbit control group recorded a decrease in the number of tubules, their overgrowth, disintegration, dystrophy and desquamation of epithelial cells. In the testes of rabbits of the experimental group, an increase in the number of tubules was noted, and no dystrophic processes were noted. The program of therapy of rabbits with gonadodystrophy proved to be quite effective. The drug «Karafand+OV» rehabilitates the structure and activates the function of testes, which as a whole normalizes reproductive ability of males and allows to offer it for use in practical veterinary medicine.