Abstract
Biologically active compound 5-nitro-4,6-dichlorobenzofuroxane (5NDCBFO) has a pronounced acaricidal, fungicidal activity and is a part of veterinary preparations used in the treatment of fungal skin diseases of animals and demodecosis caused by scabies mites of Psoroptes cuniculi species. In this connection, the determination of 5NDCBFO content is relevant. To improve the analytical performance, the determination of the analyte was carried out in a micellar system of N-decylpyridinium chloride (DPC) + dimethyl sulfoxide (DMSO) + water. Optimum conditions for spectrophotometric analysis were obtained by applying response surface methodology and Box-Benken plans. The Box-Behnken design was used to obtain the systems with the highest light absorption of 5NDCBFO. The independent factors chosen for the experiment planning were the concentration of DPX (X1), pH of the medium (X2) and the volume fraction of DMSO in the binary solvent (DMSO) (X3), the intensity of the absorption band A382 served as the target function. The optimal matrix of the system DPC + DMSO + water we found has the composition X1 = 0.94; X2 = 1; X3 = 1, or in decoded values C (DPC) = 0.087 mol/L; pH = 7.6; DMSO = 0.2. The calibration graph under optimum conditions is as follows: Y = A382= 0.046 + 8760 C (5NDCBFO) mol/L, R2 = 0.999, l = 1 cm, t = 25 C. The influence of sample components on the results of analytical determinations was studied and it was found that the spectrophotometric determination of the analyte was not hindered by their presence within wide limits. The correctness of the analytical determinations was established by the results of applying the technique to the analysis of a model mixture including a known amount of the determined substances. The consistency of the analyte contents found in relation to the quantities introduced was shown. The use of response surface methodology for the determination of 5-nitro-4,6-dichlorobenzofuroxane in biologically active preparations allowed to develop a selective and accurate technique using micellar media and spectrophotometric method of analysis.
Published Version
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