월동 배추의 추위 스트레스 반응성 ERF 유전자 발굴과 활용

Abstract

To mitigate plant freeze damage caused by extreme weather events, we need to elucidate the freeze resistance mechanism of freeze-tolerant plants through molecular biological approaches. Our research focuses on identifying the cold stress responsive, ERF(Ethylene-Responsive transcription Factor) genes that confer freeze tolerance in overwintering Chinese cabbage. Additionally, we aim to explore the molecular basis for conferring freezing tolerance to other organisms through genetic transformation. Expression of the ERF, cold stress responsive gene in overwintering Chinese cabbage, leads to the synthesis of AFP(Antifreeze Protein), which results in cellular freezing tolerance. We identified and sequenced functionally important ERF domain regions within the ERF genes, which we named BrERF1 and BrERF2. Their responsiveness to cold stress was confirmed through RT-PCR and qRT-PCR. Furthermore, we propose strategies to introduce BrERF1 and BrERF2 insertion plasmids into other organisms for transformation. Significant improvements in the freezing tolerance of transgenic organisms are expected to improve the productivity of these target organisms. In particular, the development of permanently transgenic plants using the BrERFs gene offers the possibility of increasing plant production under cold stress.