ОЦЕНКА БЕЗОПАСНОСТИ НОВОГО ПРЕПАРАТА «МТ-6» В ОСТРОМ ОПЫТЕ НА БЕЛЫХ МЫШАХ

Abstract

The article presents the results of assessing the safety of the new drug "MT-6" in an acute experiment on white mice. Experiments to assess acute toxicity were carried out in three series of experiments with intragastric, subcutaneous and intramuscular routes of administration in duplicate. The drug was administered intragastrically once after a 12-hour fasting diet in doses from 5500 to 10000 mg/kg in a volume of 0.5 ml per mouse; subcutaneously – in the dose range from 2000 to 6800 mg/kg in a volume of 0.5 ml per mouse; intramuscularly – in doses from 2000 to 6800 mg/kg in a volume of 0.25 ml per mouse. Control animals were injected with sterile isotonic sodium chloride solution in the same volumes and by the same methods. During the first day after the administration of the drug, the experimental animals were monitored continuously, then for 14 days – 2 times a day. On the basis of toxicometric data obtained during the experiment, the parameters of acute toxicity of the drug "MT-6" were established: with intragastric administration LD50 7828.5 mg/kg, with subcutaneous administration LD50 4994.1 mg/kg, with intramuscular LD50 3841.9 mg/kg. Based on this, it was concluded that the investigated drug belongs to GOST 12.1.007-76 to the 4th hazard class – low-toxic substances; according to Hodge and Sterner (1943) to the 5th hazard class – practically non-toxic substances; according to Sidorov K.K. (1977) – to the 6th class of hazard – relatively harmless substances.