국산 프로폴리스에 의한 파킨슨 방어 효과

Abstract

Parkinson"s disease is a representative neurodegenerative disease in recent century. In this study, we confirmed the effect of Korean propolis for the prevention of Parkinson"s disease using the SH-SY5Y neuroblastoma cell line. The cytotoxicity concentration of MPP+ as a factor of Parkinson’s disease for the SH-SY5Y cell line was confirmed at 200 μM. In this concentration of MPP+ showed overexpression of α-synuclein protein as a Parkinson"s marker and induced apoptosis. And propolis was inhibited apoptosis and α-synuclein expression by MPP+. The cytoprotective effect of propolis for MPP+ toxicity was appeared difference followed by treatment condition. Pre-treatment of propolis has most effectively for inhibition of apoptosis and overexpression of α-synuclein. In addition, pre-treatment with propolis increased the amount of CREB protein expression in which related cognitive function. And activation of apoptosis marker such as PARP and caspase-3 were also effectively inhibited by pre-treatment of propolis. These inactivation of various apoptosis molecules was influenced by SAPK-JNK phosphorylation. Especially, we found that Jak-Stat signaling cascade protein expression was related in MPP+ toxicity and propolis prevention for the first time. Through this study, we showed that propolis was very effectively in Parkinson’s disease prevention and clarified the molecular mechanism by propolis. Therefore, we suggested that propolis can be usefully to prevent neurodegenerative disease.