Abstract
We investigated the activity and stability of alcohol oxidase from Hansenula sp. under the electric stimulation. The activity and stability of alcohol oxidase depended on electric output voltage, stimulation time, pulse duration and pulse interval. This inactivation of the enzyme under electric stimulation could be recovered by stabilizing additives such as sugars, polymers and hydrogels. The enzyme activity retained about 52% in 10% trehalose solution under electric stimulation with 40 V and 10 min. The stabilizing of enzymes against electric stimulation showed a great potential use of enzymes in biotechnology and medical engineering fields.
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