Abstract
Purification and function of blood Plg. Act. have not been studied sufficiently. Therefore, we evaluated the separation of blood Plg. Act. using by chromatography of hydroxyapatite (H-AP). H-AP varies in each characteristics according to the size and the degree of production by heat and large particle of H-AP calcined by 1200°C are selected for the open column.Blood Plg. Act. are adsorbed on H-AP column smoosely from human plasma instead of activation of coagulation and kallikrein system. Plg. Act. was eluted out by 0.05M phosphate buffer containing 0.2M Argine and 0.3M Nacl. H-AP column adsorbed blood Plg. Act. more effective than Fibrin Celite column or Lysine Sepharose column.This method is considered an effective application in the separation and purification of blood Plg. Act. as an affinity column.
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