КОМБІНАЦІЯ ГІДРОГЕЛЮ, ТОЛУЇДИНОВОГО СИНЬОГО ТА СВІТЛА 600 НМ ДЛЯ ІНАКТИВАЦІЇ STAPHYLOCOCCUS AUREUS IN VITRO

Abstract

Skin damage is accompanied by bacterial infection of the wound. Different materials are used for accelerate tissue regeneration and minimize bacterial contamination. Also it is prevent the penetration of bacteria to damaged tissues. After the emergence of antibiotic-resistant strains of microorganisms began the search for alternative means of their inactivation. Photosensitizers are used for this purpose. Their maxima of optical absorbance are in the red and infrared regions. The use of such substances provides powerful bactericidal effects, but with low toxicity to surrounding tissues. The aim of the investigation is to determine the effectiveness of combining hydrogels with toluidine blue and irradiation by light of 600 nm to inhibit the in vitro of Staphylococcus aureus growth. Equilibrium is not formed after incubation of hydrogels with toluidine blue after 3 h in aqueous solution. During this time, 57 and 43 % of the photosensitizer is desorbed from the hydrogels PAA and D-PAA, respectively. Process rate depends on the type of polymer. Desorption of TB from D-PAA is 30% faster. Irradiation of the suspension of S. aureus by light of 600 nm reduced the CFU amount by 25 % at a dose more than 4 J/ml. Short incubation (20 min) of the PAA and D-PAA hydrogels in the bacterial suspension and light irradiation (600 nm), the amount of CFU are reduced by 33% and 15 %, respectively. Increasing the incubation time of PAA does not increase the bactericidal effect. Exposure of 80 min D-PAA with TB in a suspension of S. aureus, followed by light irradiation provides inactivation of 50 % CFU. Thus, the D-PAA system with toluidine blue in combination with 600 nm light can be used to inactivate S. aureus.