Реорганизазия астроцитов неокортекса белых крыс после 20-минутной окклюзии общих сонных артерий

Abstract

Purpose. The study is devoted to the study of the spatial organization of white rats neocortex astrocytes in the normal state and after 20-minute occlusion of the common carotid arteries (OCCA) from the standpoint of fractal formalism. Method of research. Using histological (hematoxylin and eosin staining, according to Nisl), immunohistochemical (GFAP) methods and morphometry, astrocyte bodies and processes were studied on serial frontal sections of the sensorimotor cortex in the control (falsely operated animals, n = 15) and 1, 3, 7, 14 and 30 days (n = 25) after OCCA. To describe the structure of astrocytes, fractal formalism approaches are additionally used. Fractal analysis of the obtained images was performed using the program ImageJ 1.52 (plugin FracLac 2.5). Statistical hypotheses were tested by nonparametric criteria. Results and discussion. The study showed that significant heterogeneity and heterochronicity of changes in the spatial organization of astrocytes were observed after the OCCA. More labile and reactive were distal processes of astrocytes 3−4 order. GFAP of filaments of these processes was destroyed faster in 1 day after OOS, but after 3 and 7 days the degree of its recovery (branching with filling of fractal space) was significantly higher than at the level of proximal processes. Conclusion. Thus, after acute ischemia caused by 20-minute OCCA, new data on the regularities of qualitative and quantitative changes in the spatial organization of neocortex astrocytes were obtained by fractal analysis. These, mainly adaptive-reparative, changes we refer to the mechanisms of natural protection of the nervous tissue of the brain in the reperfusion period.