Abstract
The purposeof the study was devoted to the investigation of nucleoli and related structures of the nucleus of neurons in the brain of white rats in normal and after 20-minute occlusion of the common carotid arteries.Material and methods.Structural types, tinctorial properties, number, shape, size and distribution of neuronal nuclei of the neocortex, hippocampus and amygdala in the control (falsely operated animals, n=5) and after 1, 3, 7, 14 and 30 days (n=25) after acute ischemia were studied by light (stained with hematoxilin and eosin, according to Nisl), electron (uranyl acetate and lead citrate), fluorescence (DAPI) and morphometry.Results. The study showed that neurons with one nucleolus (about 90%) prevailed in the control in all studied parts of the brain. In the postischemic period is marked by considerable heterogeneity of morphological and functional activity of nucleoli – depletion alone, activation and amplification of others. Neocortex, CA1and CA3zones of hippocampus with a large share of neurons (up to 40%) with an increased number of nucleoli were revealed after 1–7 days after ischemia. The highest content of such neurons was observed in the neocortex and CA3field of the hippocampus after 1 and 3 days. 14 days after ischemia, the number of nucleoli in neurons was restored to the level of control and remained so after 30 days.Conclusion. Thus, after acute ischemia caused by 20-minute occlusion of the common carotid arteries, qualitative and quantitative changes in nucleoli were revealed. Within 7 days after reperfusion, there was an "adaptive-reparative shift" in the chromosomal balance and the activity of the existing nucleolar organizers of the preserved neurons increased. These changes we refer to the mechanisms of natural protection of the nervous tissue of the brain in the reperfusion period.
Highlights
Structural types, tinctorial properties, number, shape, size and distribution of neuronal nuclei of the neocortex, hippocampus and amygdala in the control and after 1, 3, 7, 14 and 30 days (n=25) after acute ischemia were studied by light, electron, fluorescence (DAPI) and morphometry
In the postischemic period is marked by considerable heterogeneity of morphological and functional activity of nucleoli – depletion alone, activation and amplification of others
CA1 and CA3 zones of hippocampus with a large share of neurons with an increased number of nucleoli were revealed after 1–7 days after ischemia
Summary
После острой ишемии, вызванной 20-минутной окклюзией общих сонных артерий, выявлены качественные и количественные изменения ядрышек. В течение 7 сут после реперфузии происходил «адаптационно-репаративный сдвиг» в хромосомном балансе и повышалась активность имеющихся ядрышковых организаторов сохранившихся нейронов. Эти изменения мы относим к механизмам естественной защиты нервной ткани головного мозга в реперфузионном периоде. Ключевые слова: острая ишемия, неокортекс, гиппокамп, амигдала, нейроны, ядра, ядрышки, крысы Wistar. The purpose of the study was devoted to the investigation of nucleoli and related structures of the nucleus of neurons in the brain of white rats in normal and after 20-minute occlusion of the common carotid arteries
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