Abstract
Microbial xylanases are widely used across various industries, making the search for new microorganisms capable of producing these enzymes a highly relevant task. A source for isolating strains with xylanolytic activity is plant-based materials rich in hemicelluloses, such as lignocellulosic biomass. In the Kaliningrad region, such materials include the meal of industrial hemp seeds (Cannabis sativa), seed hulls and seeds of white lupine (Lupinus albus), unroasted soybean hulls, roasted soybean hulls, granulated soybean hulls, and seeds of common horse chestnut (Aesculus hippocastanum). Among the 23 bacterial isolates obtained from the mentioned lignocellulosic materials, the highest xylanolytic activity was observed in nine strains. Based on morphological and staining characteristics, these strains were identified as Gram-positive spore-forming rods. Molecular genetic identification using the 16S rRNA method revealed that the strains belong to the species Peribacillus acanthi, Bacillus cereus, Bacillus zanthoxyli, and Bacillus badius. The identified microbial strains have the potential to be used as industrial producers of highly active xylanases, enabling the biodestruction of lignocellulosic biomass to produce valuable products.
Published Version
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