Влияние нефрэктомии на реактивность артерий крыс линии Wistar-Kyoto

Abstract

The effect of nephrectomy on the Wistar Kyoto rat superior mesenteric artery (SMA) and internal carotid artery (ICA) segments constriction and dilatation ability was studied under isometric conditions using myograph. 5/6 nephrectomy (5/6 Nx) in the rats 3 months old was used as a model of the chronic kidney disease. Dilatation of the phenylephrine-precontracted segments was induced by acetylcholine (AСh) or sodium nitroprusside (SNP) in the absence and presence of potassium channels blockers (tetraethylammonium, glibenclamide) or methylene blue, a guanylyl-cyclase inhibitor. The phenylephrine-induced contraction was increased in the SMA but not in the ICA from 5/6 Nx rats. This reaction was not changed by the blockers and by inhibitor. The AСh-evoked reaction often had two phases. AСh-evoked dilatation phase of the reaction of the both arteries from 5/6 Nx rats have shown no difference from control (C) and was diminished by tetraethylammonium (in С and 5/6 Nx rats) and by methylene blue (in С rats). In 5/6 Nx rats, AСh-evoked constriction phase of the reaction in the absence of the blockers and inhibitor was significantly larger compared to С rats only in SMA, but in the absence of tetraethylammonium, it was significantly larger in both arteries. Glibenclamide diminished AСh-induced constriction of SMA from 5/6 Nx rats, while methylene blue diminished this constriction of ICA from control rats only. We conclude that the chronic kidney disease may increase endothelium dependent and independent vasoconstriction and may change signal pathways of this constriction, which may differ in the arteries of different types.