Abstract
The aim of the study was to test the complex drug entrikima, which includes: enrofloxacin, trimethoprim and tilmicosin phosphate, on lambs and goats, in farms with mycoplasmosis problems. Before the start of the drug testing, 3 samples from each animal species (6 in total) were taken from diseased lambs and goats in the form of a smear from the nasal passages (during life), as well as after their death from the lungs and articular fluid. Bacteriological studies of a nasal smear isolated P. multocida isolates from 6 samples in 6, and S. isolates were found in 3 samples. sp. hamolisierend, in 2 samples of isolates P. haemolytica and M. mycoides subsp. mycoides. When examining the light corpses of fallen lambs and goats, 3 samples were also taken from each animal species. At the same time, P. multocida isolates were isolated from 6 samples in 6, S. isolates were found in 4 samples. sp. hamolisierend, in 3 samples of P. isolates. haemolytica and M. agalactiae and in 2 M. mycoides subsp. mycoides. In the study of articular fluid from the corpses of fallen lambs and goats, 3 samples were also taken from each animal species. At the same time, isolates were isolated in 6 samples out of 6. - S. sp. hamolisierend, isolates were found in 4 samples: P. haemolytica, M. agalactiae и M. mycoides subsp. mycoides. To confirm the diagnosis obtained by bacteriological studies, a molecular genetic diagnostic method (PCR) was also used. In PCR, the results obtained during bacteriological studies of the lungs were confirmed. The genomes of pathogens: M. mycoides subsp. mycoides, M. agalactiae P. haemolytica and S. sp. hamolisierend were detected in all 6 samples. For the treatment of lambs and goats, a 5% solution of entrikim was used orally at a dose of 0.5 cm3 / 1 kg of body weight twice a day for 7 consecutive days. 3 days after the end of the course of treatment, a nasal smear was taken for complex laboratory studies (bacteriological and molecular genetic). PCR studies have established that the applied therapy did not fully have a positive effect on the release of the organism of young cattle from certain types of mycoplasmas, in particular, the M. agalactiae genome was detected in one sample out of six (16.6%), as well as in two samples out of six genomes of P. multocida and S. sp. Hamolisierend (33.3%).
Published Version
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