ФІЛОГЕНЕТИЧНИЙ АНАЛІЗ ІЗОЛЯТІВ ВІРУСУ ЖОВТОЇ МОЗАЇКИ КВАСОЛІ ІЗГЛАДІОЛУСІВВУКРАЇНІ

Abstract

Bean yellow mosaic virus (BYMV) is the one of most prevalent and harmful viruses infecting gladiolus plants worldwide. The aim of the study was to perform phylogenetic analysis of two Ukrainian BYMV isolates from gladioli grown in different regions of Ukraine and determine phylogenetic relationships of the isolates to other BYMVs. Visual diagnostics, enzyme-linked immunosorbent assay in DAS-ELISA modification, total RNA extraction, RT-PCR, phylogenetic analysis and statistical data analysis were used in this research. The results of the studies showed that gladioli of variety Pamyat’ (Poltava region, 2018) and var. Galyna Zelenobirska (Kyiv region, 2020) with symptoms of chlorotic stripes and mosaics on the leaves, flower color breaks are infected by BYMV. Fragments of the CP gene sequence of Ukrainian gladiolus isolates of bean yellow mosaic virus, named GlMP-18 (MK416160) and BYMV-Gl-SV-20 (MZ286966) were sequenced and deposited to the NCBI GenBank. Nucleotide sequences of these isolates corresponding to 578 nt of the coat protein gene (CP) located at the position 8727–9305 of the viral genome and amino acid sequences were compared with 40 known BYMV isolates/strains. Phylogenetic analysis demonstrated that GlMP-18 and BYMV-Gl-SV-20 have identity of nucleotide sequence 100% and amino acid sequence 100% with each other. Both Ukrainian isolates clustered with the Monocot group. The identity of the СР gene sequences of the two gladiolus isolates GlMP-18 and BYMV-Gl-SV-20 from geographically remote regions of the country and in different years of selection indicates a common origin of isolates and probable their distribution of planting material.