Особливості застосування екстракту алое у технології культивування герпесвірусів

Abstract

In the article there are studied peculiarities of aloe extract application in the cultivation technology of herpes viruses on the re-cultivating culture of testicular piglets cells. We were faced with the following tasks: to select a cell culture and aloe extract concentration to determine the toxic dose of the drug; to study the manifestation of the cytopathic effect in cell cultures infected with herpesvirus at different drug concentrations ; to determine infectious virus activity after its passage on the cells culture with the use of aloe extract. The work was carried out at the faculty of veterinary medicine, in the educational-research laboratory of epizootology department of microbiology, pharmacology and epizootology of the Zhytomyr National Agroecological University. The research materials included: the re-cultivating culture of testicular piglets, horse herpes virus of the first type and sterile aloe extract. Cultivation of crops, infection with the virus and determination of its concentration after contact with culture and the introduction of aloe extract were carried out under sterile conditions according to the generally accepted methods. The results were recorded daily, paying attention to the environment color changing, the integrity of the monolayer and the presence of cells in the medium. Along with the infected cells we were observing additional, uninfected, cultures of cells. The Incubation process was stopped at the destruction of 90-100% cell monolayer. As a result of this work, it is proved that the aloe extract in a dose of 0.14-0.28 mg per cm³ of growth medium allows to observe for the 2nd day of replanting a solid dense monolayer in the mattress without color changing , as well as enhanced growth and regeneration of cells and the metabolism of the epithelial tissue of the re-cultivating culture of testicular piglets, which makes it possible to shorten the time to grow a new monolayer for further virological and immunological studies. The use of this drug helps to increase cells concentration in the mattresses, which, in turn, can increase the concentration of the virus to 1-2 log2 for the use of similar amounts of growth medium without aloe extract. Such modified way to increase the biomass of cells is suitable for the further multiplication of viruses and the production of diagnostic antigens with lower economic costs. The use of this methodical approach will allow to solve a number of tasks related with accumulation of viruses and the production of drugs for the diagnosis and prevention of viral infections.