ИММУНОФЛУОРЕСЦЕНТНАЯ РЕАКЦИЯ АГГЛЮТИНАЦИИ ДЛЯ ДИАГНОСТИКИ АССОЦИИРОВАННЫХ ИНФЕКЦИЙ СВИНЕЙ

Abstract

The purpose of the research was to develop a diagnostic algorithm for identifying associated viral-bacterial infections in pigs. The studies were carried out on a farm characterized by the presence of multiple abscesses, laminitis, dermatitis and respiratory pathology in pigs. To detect associated PRRSV infections with bacteria isolated from sick animals, an immunofluorescent agglutination reaction with a fluorescently labeled antigen was used. Antibodies to PRRS were determined by ELISA using the PRRS-SEROTEST reagent kit. The research revealed the presence of antibodies to the PRRS virus in the herd. The examined livestock had an associated infection with Streptococcus suis, Escherichia coli, Trueperella pyogenes, which were not dependent on PRRS. The proposed serological diagnostic algorithm based on: isolating bacterial cultures from sick animals, conducting agglutination tests with antigens from these bacteria, comparing the levels and presence of antibodies to various viral and bacterial agents. This methodological approach allowed us to establish the relationship between Trueperella pyogenes, Streptococcus suis and E.coli and exclude the contribution of PRRSV to this associated infection. The versatility of the proposed methodology is promising for diagnosing a wide range of bacterial and viral associated and mono infections.