Abstract
Field and micropropagated plants were tested for the presence of RBDV by DAS-ELISA and PCR. For in vitro therapy, plants with a proven absence of ArMV, ApMV, RpRSV, TBRV, TomRSV, SLRV, CMV, TRSV and infected with Raspberry bushy dwarf virus (RBDV) were selected.Virazole and salicylic acid at concentrations of 30, 50 and 70 mg/l were used as antiviral preparation.Low phytotoxicity of salicylic acid and high phytotoxicity of virazole were established. Long-term use of virazole, at concentrations of 50 and 70 mg/l, by the end of the 3rd passage leads to the complete death of microshoots. The studies were carried out within the framework of the project „Development of biotechnological methods (in vitro and ex vitro) obtaining virus-free material from the raspberry bushy dwarf virus” in order to increase the productivity of industrial raspberry plantations.
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