Abstract
This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city. Screening of L. lactis ssp. Lactis and isolated from the intestines fish and raw milk was performed in well diffusion method. The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration. The concentrated bacteriocin was purified using the gel filtration column and Sephacryl S-200. The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification , The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively. The carbohydrate content of purified bacteriocin from L. lactis ssp. lactis (Lc4) isolate was 6.02% with a molecular weight of 6310 Dalton. The results showed that purified bacteriocin retained its inhibitory activity at pH 2-10 and showed the highest inhibition at pH 4-6 and lost at pH 12. The purified bacteriocin was characterized by thermal stability. It retained its effectiveness when exposed to 40, 60, 80, 100°C for 30, 15, 5 minutes and 120°C for 15.5 minutes and lost 50% of its effectiveness when exposed to 120°C for 30 minutes. Results The purified bacteriocin was effectively retained when treated with enzyme pepsin and trypsin of 37°C for one hour and at pH 7.
Highlights
This study aimed to study the inhibition activity of purified bacteriocin produced from the local isolation Lactococcuslactis ssp. lactis against pathogenic bacteria species isolated from clinical samples in some hospitals Baghdad city
The results showed that L. lactis ssp. lactis (Lc4) was the most efficient isolate in producing the bacteriocin as well observed inhibitory activity the increased that companied with the concentration, the concentration of the twice filtrate was better in obtaining higher inhibition diameters compared to the one-fold concentration
The results showed the high inhibitory activity of the purified bacteriocin after the purification against the positive and negative bacteria of the Gram stain under study compared to the one-fold concentration and two-fold before purification, The diameters of the inhibition zones after gel-filtering of the purified bacteriocin reached S. aureus, S. epidermidis, P. aeruginosa, E. coli, E. clocae and S. marcescens (23, 25, 26, 20, 22 and 28) Mm respectively
Summary
ثٞعبؽخ ٓؾِ ٍٞداسئ كٞعلبد اُجٞربعٝ ّٞ٤ثزشً٤ض ٞٓ 0.5لاس.١ ه٤غذ ثؼذٛب اُلؼبُ٤خ اُزضج٤ط٤خ ُِؤْ أُلظُٞخ ثطش٣وخ الأٗزشبس ك ٢اُؾلش ،فر ًبٗذ اُلؼبُ٤خ اُزضج٤ط٤خ ٓزٔشًضح ك ٢اُؤخ اُضبٗ٤خ ،آب اُؤخ الأ ٠ُٝكٌبٗذ خبُ٤خ روش٣جب ٖٓ اُلؼبُ٤خ رغب ٙاُجٌزش٣ب الاخزجبس٣خٝ ،عٔؼذ أعضاء اُؤخ اُضبٗ٤خ ٝسًضد ٓشح أخش ٟصْ اػ٤ذ اػبكزٜب اُ ٠ػٔٞد اُزشش٤ؼ اُٜلآٗ ٢لغ ٚك ٢خطٞح صبٗ٤خ ٝرؾذ ظشٝف ٓٔبصِخ ُِلظَ الأ ،ٍٝرْ ٖٓ خلاٍ ٛز ٙاُخطٞح اُؾظ ٍٞػِ ٠هٔخ ٝاؽذح رطبثوذ ٓغ اُؤخ اُضبٗ٤خ أُزؾظَ ػِىٜب ك ٢اُخطٞح الأٔٓ ،٠ُٝب ٣ذٍ ػِٗ ٠وبٝح اُجشٝرٝ ٖ٤إٔ أُٞاد اُجشٝر٤٘٤خ أُضجطخ هذ سشؾذ خلاٍ الاعضاء اُ٘بكزح ،كٔ٤ب اخزِق رشً٤ض اُجشٝر ٖ٤ك ٢الأعضاء أُغزشدح ٖٓ اُزشش٤ؼ اُٜلآ ٢رجؼبً لاخزلاف رشً٤ض أُٞاد أُضجطخ ،فر أػطذ كؼبُ٤خ رضج٤ط٤خ ػبُ٤خ ٝثأهطبس رضج٤ؾ ٓزلبٝرخ رغب ٙاُجٌزش٣ب الاخزجبس٣خٝ ،عش ٟأعزؼٔبٍ اُزشؽ َ٤اٌُٜشثبئ ٢ثٜلاّ ٓزؼذد الأًش٣لآب٣ذ ٝثٞعٞد اُؼٞآَ أُبعخخ ُِزأًذ ٖٓ دهخ ػِٔ٤خ اُز٘و٤خ ُِجشٝر٘٤بد أُلظُٞخ ،ار اٝػؾذ اُ٘زبئظ أُغزؾظَ ػِٜ٤ب إٔ اعزٔشاس ػِٔ٤خ اُز٘و٤خ ُِشاشؼ ٣ؤد ١فُ ٠ر٘بهض ك٢ ٓوذاس اُؾضّ اُجشٝر٤٘٤خٔٓ ،ب ٣ؤًذ ٗغبػ ػِٔ٤خ اُز٘و٤خ ك ٢اُؾظ ٍٞػِ ٠ثشٝرٝ ٖ٤ثأػِ ٠دسعخ ٖٓ دسعبد اُ٘وبٝح ُ٤زْ رٞط٤ق رُي اُجشٝرٝ ٖ٤روذ٣ش كؼبُ٤ز ٚاُزضج٤ط٤خ ثظٞسح ده٤وخ ثبُشعٞع اُ ٠ه٤بط اُلؼبُ٤خ اُزضج٤ط٤خ ُلاعضاء أُغزشدح ٖٓ ٛلاّ ٣ٝ ephacryl S-200لاؽع ٖٓ خلاٍ (اُشٌَ )4ٝ 3 ،أصد٣بد اهطبس ٓ٘بؽن اُزضج٤ؾ P.
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