Abstract
Infectious keratitis poses a significant diagnostic challenge due to the diverse range of causative agents involved, including difficult-to-cultivate and atypical pathogens that are often missed by standard identification methods. Purpose. This study aims to present clinical cases where the successful preliminary verification of difficult-to-cultivate pathogens in infectious keratitis was achieved using scanning electron microscopy (SEM) and lantanoid staining. Material and methods. The article describes two cases of infectious keratitis in patients with a special status of the ocular surface, where conventional bacteriological analysis failed to identify the causative agent. Results. The optimal approach to the treatment of patients was chosen taking into account the information obtained from SEM with lanthanide contrast. Conclusion. The results demonstrate the potential of lanthanide staining and SEM as an additional tool for the identification of atypical pathogens in patients with keratitis and concomitant eye diseases. Key words: ocular surface, cornea, keratitis, scanning electron microscopy, microbiome, bacteria, fungi
Published Version
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