Abstract
본 연구는 섬유소분해능이 우수한 L. japonicus의 균사생육에 미치는 중요 인자에 대한 배양학적 특성을 규명하여 인공재배 및 균사체 배양을 이용한 섬유소분해효소의 생산을 높일 수 있는 기초자료를 얻고자 실험을 수행하였다. 그 결과, 균사체 배양의 최적온도는 <TEX>$30^{\circ}C$</TEX>이었으며, 최적 pH 는 6.0이었다. 균사생장에 적합한 배지는 glucose peptone 배지, malt yeast extract 배지, yeast malt peptone 배지, potato dextrose 배지, Hennerberg 배지 등으로 조사되었다. 균사생장에 가장 양호한 탄소원은 dextrose 였으며, 최적농도는 1.2%이었다. 또한 질소원으로서 균사생장에 가장 양호한 유기, 무기질소원, 아미노산의 종류 및 최적농도는 각각 yeast extract(1.7%), <TEX>$(NH_4)_2HPO_4(0.2%)$</TEX>, glutamine(0.2%) 이었다. 균사생장에 양호한 무기염류는 <TEX>$Al_2(SO_4)_3{\cdot}14H_2O$</TEX>인 것으로 조사되었으며 최적농도는 0.1 M 이었다. This study was carried out to obtain the basic data on artificial culture of Lampteromyces japonicus. Favorable media for mycerial growth were glucose peptone medium, malt yeast extract, yeast malt peptone, potato dextrose medium. The optimum conditions for the mycelial growth were <TEX>$30^{\circ}C$</TEX> and pH 6.0. Dextrose as a carbon source was favorable to mycelial growth. The optimal dextrose concentration was 1.2%. As nitrogen sources, yeast extract, <TEX>$(NH_4)_2HPO_4$</TEX> and 0.2% for glutamine. The mineral salts of <TEX>$Al_2(SO_4)_3{\cdot}14H_2O$</TEX> were effective and the optimal concentration was 0.1 M.
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