Performance of Vegetative Growth and Artificial Fruit Body Formation of Hypsizygus marmoreus in Bangladesh

Abstract

Hypsizygus marmoreus is an edible and medicinal mushroom belongs to Basidiomycetes. The optimal culture conditions for the vegetative growth of brown and white strains of H. marmoreus were investigated to obtain suitable farming condition. Temperature suitable and unsuitable for vegetative growth was obtained at 20 and 35oC, respectively. The both strains of H. mermoreus love medium temperature and the mycelial growth was well developed at 20-25oC. This mushroom prefer pH ranging 6-8 for mycelial growth. The highest and lowest mycelial growth was found at pH 7 and 5 of both strains, respectively. Among 5 different carbon sources, sucrose was the best, whereas, maltose and lactose were the worst. The most suitable nitrogen sources were CaNO3 (in brown strain 65.6 mm and in white strain 56.3 mm) and the most unsuitable was NH_4H_2PO_4 (in brown strain 44.02 mm and in white strain 40.2 mm). Five different culture media were used to screen the optimal mycelial growth of H. marmoreus. PDA and YM (yeast-malt extract) were the most suitable and Czapek dox and Hennerberg were the most unfavorable for vegetative growth, whereas, glucose peptone was moderately suitable. The highest and lowest vegetative growth was observed in media prepared with mango (Mangifera indica) and koroi (Albizia procera) sawdust, respectively. However, for both strains, 3 weeks were required to complete mycelium running in mango sawdust, whereas, 4 weeks were spent to get 100% mycelium running in mahagoni (Swietenia mahagoni) and koroi sawdust. The premordia and fruiting bodies of H. marmoreus were formed in the mango sawdust medium sooner. The fruiting bodies of brown strain were formed earlier than white strain after occurrence of primordia. In case of limiting ventilation, the stem development was found to be good but no cap was seen to be formed. Ventilation influenced the growth and development of fruit body of H. marmoreus. Therefore, it could be said that physiochemical requirement for the vegetative growth is strictly considerable and results focused in this paper could be followed by farmers to scale up farming of H. marmoreus in Bangladesh.