Исследование наличия ДНК папилломавирусов человека (ВПЧ) в бытовых стоках

Abstract

Human papillomavirus (HPV) appears to be significant danger for mankind because of having very high widespread and might revealing the pandemy of papillomatoses. To the present time, about 200 types of HPV have been found, 40 types from which announced as high-risk cancerogenic. It was learned that papillomaviruses are very resistant to impacts of environmetal factors because they posseses many possibilities for long surviving in polluted water conditions. Nevertheless, the infection of HPV through the contact with contaminated water areas still does not to be prove with strong scientific data, foreign scientists are seriously bothering about facts of finding of DNA of human papillomaviruses in sewage water and in different deteriorated water objects. Our opinion is based on the necessity of the comprehensive investigation of the presence of the high-risk oncogenic types of HPV in sewage water. The present study is devoted for the first time to the data of finding of DNA of several high-risk oncogenic HPV in sewage water. Three pairs of primers were used in the study, between them 2 pairs of universal primers (GP5+/6+) which were very specific to nucleic sequences encoded the main structural element of the HPV capsid, the “late” protein L1 (HPV6 L1, HPV11 L1 and HPV16 L1). As the result, the presence of DNA of high-risk oncogenic papillomaviruses of types 6 and 16 was shown in sewage water. Further, the scope of investigations directed to finding of the presence of HPV components is planned to be arranged not only for sewage water but for other water areas of Irkutsk Region of East Siberia as well around water areas of the Lake Baikal.