Abstract
In general pathology of dogs the enterovirus infections of puppies occupy a leading place, and the special disturbance is caused by illnesses of the mixed etiology, that run across with off type the display of clinical signs. The goal of the work was to identify the hematological and biochemical change in the blood of dogs sick with coronavirus enteritis. To confirm canine coronavirus was used VetExpert rapid tests in the veterinary clinics of Zhytomir and as well as positive results of private laboratory Bald Ltd. (Kyiv). Hematological and biochemical researches were made by biochemical analyzer BioChem SA with chemical of High Technology Inc. (USA). Where analyzed in blood amount of RBC, WBC, leucogram, hemoglobin, hematocrit, speed of erythrocyte sedimentation by electronic-automatic method. In serum blood detected common protein by biuretic method, urea by fermentation method, creatinine by Jaffe method, common calcium by comlexone Arsonaso-3, nonorganic phosphorus by phosphomolibdate method, glucose by fermentation method. Activity of Asparagine and Alanine aminotranpedase (AsAT and AlAT) by method of Reitmann-Frenkel, alkaline phosphatase be kinetic method. In 2014–2016 were found and confirmed diagnosis coronavirus enteritis in 10 dogs. The temperature of their body was under the normal condition. In relation to morphological and biochemical indexes then there is reduction to the amount of leucocytes on 46%, erythrocytes on 5% and lymphocytes on 13% and by reduction of hematocrit on 5% below minimum limit of norm, increase of activity alkaline phosphatase and increase speed of settling of red corpuscles in blood and marked the change of leucocytes formula to the right for an account to the increases of amount of the segmented nuclear forms of neutrophil. For dogs at this pathology a liver is struck, to what reduction of level general testifies squirrel on 23 % comparatively with the minimum values of norm. Perspective is a further study of the state of blood, namely – leucocytes profile for deeper research of pathological processes in the organism of dogs, patients with coronavirus enteritis.
Highlights
Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies named after S.Z
In general pathology of dogs the enterovirus infections of puppies occupy a leading place, and the special disturbance is caused by illnesses of the mixed etiology, that run across with off type the display of clinical signs
In relation to morphological and biochemical indexes there is reduction to the amount of leucocytes on 46%, erythrocytes on 5% and lymphocytes on 13% and by reduction of hematocrit on 5% below minimum limit of norm, increase of activity alkaline phosphatase and increase speed of settling of red corpuscles in blood and marked the change of leucocytes formula to the right for an account to the increases of amount of the segmented nuclear forms of neutrophil
Summary
Робота виконувалась на факультеті ветеринарної медицини Житомирського національного агроекологічного університету (ЖНАЕУ), а також у ветеринарних клініках міста Житомира: приватних ветеринарних клініках «Багіра» і «Доктор–Zoo», навчальнонауково-виробничій клініці ветеринарної медицини факультету ветеринарної медицини ЖНАЕУ та в міській державній лікарні ветеринарної медицини м. Житомира (період з 2014 по 2016 рік) на породних і безпородних собаках. Діагностичні дослідження на підтвердження коронавірусного ентериту проводили за допомогою експрес-тестів VetExpert та в приватній ветеринарній лабораторії ТОВ «Бальд» Гематологічні та біохімічні дослідження проводили за допомогою біохімічного аналізатора BioChem SA із застосуванням реактивів фірми High Tehnology, Inc. Підрахунок еритроцитів, лейкоцитів та лейкограми, гемоглобіну, гематокритної величини та швидкість осідання еритроцитів (ШОЕ) проводили електронноавтоматичним методом. Активність аспарагінової та аланінової амінотранспептидаз (АсАТ і АлАТ) визначали методом Райтмана-Френкеля, лужної фосфатази – кінетично (Sukmanskyi, 2009). Цифрові дані обробляли біометрично загальноприйнятими методами варіаційної статистики з використанням комп’ютерних програм Statistika 6.0 та Microsoft Exсel 2007 (Kochetov et al, 2012)
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