Abstract
The effects of inorganic nitrogen sources such as KNO<TEX>$_3$</TEX> and NH<TEX>$_4$</TEX> NO<TEX>$_3$</TEX> on cell growth and production of chlorogenic acid and eleutheroside E derivative were investigated in 5L bioreactor cultures of Eleutherococcus senticosus. The cell growth in the 1/2MS medium containing 15mMKNO<TEX>$_{3}$</TEX>. The fresh weight of cells harvested from bioreactor was affected by the concentration ratio of NO<TEX>$_3$</TEX><TEX>$^{[-10]}$</TEX> and NH<TEX>$_4$</TEX><TEX>$^{+}$</TEX> in culture medium. At the viewpoint of secondary metabolite production, the production of chlorogenic acid was affected by the concentration of NH<TEX>$_4$</TEX><TEX>$^{+}$</TEX> in the culture medium, but not by the total concentration of nitrogen sources in the culture medium. Futhermore, eleutheroside E derivative production was also affected by the concentration ratio of NO<TEX>$_3$</TEX><TEX>$^{[-10]}$</TEX> and NH<TEX>$_4$</TEX><TEX>$^{+}$</TEX> in the culture medium. Base on those results, it is suggested that cell growth and production of secondary metabolite(chlorogenic acid and eleutheroside E derivative) could be manipulated by controlling the total concentration of nitrogen sources and the concentration ratio of NO<TEX>$_3$</TEX><TEX>$^{[-10]}$</TEX> and NH<TEX>$_4$</TEX><TEX>$^{+}$</TEX> in the culture medium. medium.
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