ПАРАМЕТРЫ НЕТОЗА У ПАЦИЕНТОВ С СЕЛЕКТИВНЫМ ДЕФИЦИТОМ ИММУНОГЛОБУЛИНА А

Abstract

Objective. To study NETosis parameters in patients with selective immunoglobulin A deficiency. Material and methods. We studied the features of NETosis in peripheral blood of 46 patients (21 males and 25 females, mean age 37±12.7 years) with a confirmed diagnosis of selective immunoglobulin A deficiency. Results. Аctivation of NETosis (NET30SP, NET30ST, NET150SP and NET150ST, р<0.001) was found in patients with selective IgA deficiency relative to the comparison group. The maximum degree of increase in leukocyte NETosis in patients was observed during short-term incubation of cells (NET30SP, NET30ST). The relationship between the level of NETosis and apoptosis of neutrophils has been revealed. The patients with a simultaneous activation of NETosis and superoxide anion production by neutrophils showed a higher incidence of combined clinical manifestations compared to patients with an isolated increase of NETosis (χ2=9.19; р=0.002). Conclusion. This study demonstrated that in patients with selective immunoglobulin A deficiency during remission of infectious inflammatory diseases, the ability of blood neutrophils to form extracellular networks in combination with the activation of other manifestations of neutrophils reactivity – superoxide anion-producing function and apoptosis – is increased.