Особенности влияния галогенидов натрия и калия на рост культуры Bifidobacterium bifidum

Abstract

Halide ions are a type of anions that are commonly found in the environment, have important physiological functions, and are used in industry. However, their high content is harmful to the environment and human health, largely depending on the status of the endogenous microbiota. The aim of this work is to research the features of the influence of halides on Bifidobacterium bifidum growth in vitro. The dependence of biomass accumulation in the stationary phase on the content of sodium and potassium halides in thioglycol medium at different concentrations (0,001, 0,01, 0,1 and 1 M) was studied. Empirical values were fitted with dose-response model curves. Two-factor PERMANOVA was shown that the shape of the curves significantly (p<0,01) depends on the type of anion, type of cation, salt concentration and the interaction of factors “type of anion / salt concentration”. Moreover, the biomass of bacteria is often lower in the presence of potassium salts than similar sodium ones. As a result, model curves for different salts has various form (logistics, exponential and hyperbolic). Fluorides and chlorides inhibited biomass growth at all concentrations compared to control. Bromides and iodides at 0,001–0,01 M stimulated the accumulation of biomass to 20–50%. This effect can be used in the food industry, agriculture and healthcare. At a concentration of 0,001 M, a significant regression relation was shown between the atomic mass of the halogen and the fitted curve value of biomass. This dependence probably demonstrates effect atomic radius or/and electronegativity halogens for their penetration into the cell and interaction with cellular targets. The result dependencies do not correspond with state sanitary standards for halide ions. These regulations for fluorides (~10–4 M) and chlorides (10–2 M) roughly match to the survival limit of bifidobacteria. However, more strong limits for bromides (~10–6 M) and iodides (~10–6 M) do not correlate with the biological response of bifidobacteria. In addition, the sanitarian standards halogenids do not take into account the modifying effect of cations which can be important.