Abstract
The whole genome sequence of unicellular cyanobacterium Synechocystis sp. strain PCC 6803 was completed in 1996 and nitrogen fixable filamentous cyanobacterium Anabaena sp. strain PCC 7120 is almost completed in our Kazusa DNA Research Institute. We initiated characterization of two organisms from a proteomic viewpoint by exploiting two-dimensional gel electrophoresis coupled with N-terminal protein sequencing. As a result, we succeeded in linking 347 protein spots on two-dimensional gels with the genes encoded on the genome of both species. These results provide us the “protein-gene linkage maps”of two cyanobacteria, which are highly beneficial for functional analysis of cyanobacteria at protein level. Compared two cyanobacterial proteomes with their whole genome sequences, we can extract many items of information concerning their surrounding nucleotide sequences of translation initiation site, rare initiation colons, post-translational processings (including translation initiator methionine processing, signal peptide processing and etc.) concerning to many functionally known and unknown cyanobacterial proteins. In consequence, comparative genomics together with comparative proteomics will serve us very valuable information comprehensive understanding of the molecular basis of cyanobacterial life.
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