Abstract
In this work, we have studied the effect of graphene oxide on the regenerants of the white poplar × aspen hybrid when they were transferred to non-sterile greenhouse conditions during clonal micropropagation. It was shown that a suspension of the nanomaterial at a concentration of 1.5 μg / L stimulated the growth of plant height in the hybrid (white poplar × aspen), but also increased the number of wilted leaves. An increase in the concentration of nanomaterial to 3 μg / L caused a negative effect on seedlings. The number of surviving shoots decreased by 17% relative to the control, plant height decreased by 25%. A decrease in the number of leaves and adapted microclones was also observed: 15% of plants adapted against 55% in the control group. The general condition of the seedlings of this group was assessed only by 3 points out of 5. Analysis of binaccumulation of graphene oxide in plant organs has not showed pronounced foci of nanomaterial accumulation. Thus, graphene oxide had a multidirectional effect on the development of seedlings of the hybrid (white poplar × aspen). The results of the study can be used in assessing the environmental safety of graphene-like materials in relation to plants, as well as for improving the biotechnology of clonal micropropagation of forest plantations
Published Version
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